Analytical Biochemistry 1985-08-15

Determination of alpha-amanitin and beta-amanitin in human biological fluids by high-performance liquid chromatography.

F Jehl, C Gallion, P Birckel, A Jaeger, F Flesch, R Minck

Index: Anal. Biochem. 149(1) , 35-42, (1985)

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Abstract

A high-performance liquid chromatographic assay of alpha-amanitin and beta-amanitin in human serum, urine, or stomach washings is described. Sample preparation involves a chemical step with deproteinization and organic solvent treatment, and a selective cleanup and concentration step on reversed-phase prepacked cartridges. Separations are performed on a reversed-phase analytical column under isocratic conditions with uv detection at 280 nm. The method allows the quantitation of alpha- and beta-amanitin separately with a detection limit of 10 ng/ml for both toxins.