Journal of the American Chemical Society 2005-06-08

Nonactin biosynthesis: the product of the resistance gene degrades nonactin stereospecifically to form homochiral nonactate dimers.

James E Cox, Nigel D Priestley

Index: J. Am. Chem. Soc. 127(22) , 7976-7, (2005)

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Abstract

Heterologous expression of the tetranactin resistance gene nonR in E. coli gave a his6-tagged protein that catalyzed the stereoselective hydrolysis of the chiral macrotetrolide antibiotic nonactin into equivalent, chiral, inactive nonactate dimer species. No trimeric nonactate was produced in the reaction.