PNAS 2015-01-06

Dipeptides catalyze rapid peptide exchange on MHC class I molecules.

Sunil Kumar Saini, Heiko Schuster, Venkat Raman Ramnarayan, Hans-Georg Rammensee, Stefan Stevanović, Sebastian Springer

Index: Proc. Natl. Acad. Sci. U. S. A. 112(1) , 202-7, (2015)

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Abstract

Peptide ligand selection by MHC class I molecules, which occurs by iterative optimization, is the centerpiece of immunodominance in antiviral and antitumor immune responses. For its understanding, the molecular mechanisms of peptide binding and dissociation by class I molecules must be elucidated. To this end, we have investigated dipeptides that bind to the F pocket of class I molecules. We find that they accelerate the dissociation of prebound peptides of both low and high affinity, suggesting a mechanism of action for the peptide-exchange chaperone tapasin. Peptide exchange on class I molecules also has practical uses in epitope discovery and T-cell monitoring.