A(4a) = 46.8 G, A(4b) = 45.8, given the assumptions in section V. Although this difference is small (2%), we believe it to be real. Analyses of NADH spectra obtained with other dyes give similar results.29 A slightly nonplanar geometry of the nicotinamide ring in NADH'. brought about by a stacking interaction with the adenine ring could be responsible. ... Acknowledgment. This work was performed at the national NMR facility at the University of Groningen, supported by ...