Journal of Chromatography A 2009-01-01

Comparative application of microwave, ultrasonication, ultracentrifugation and conventional heating for preparation of sample as dinitrophenyl derivative for direct enantioseparation of certain amino alcohols and 1-amino-2-propanol from vitamin B12hydrolysate on α1-acid glycoprotein and β-cyclodextrin columns

Ravi Bhushan, Rajender Kumar, Ravi Bhushan, Rajender Kumar

Index: J. Chromatogr. A. 1216(45) , 7941-5, (2009)

Full Text: HTML

Abstract

Taking into account the structural similarities of amino alcohols with amino acids and in order to reduce time for derivatization unconventional approaches viz. microwave irradiation, ultrasonication and ultra centrifugation were applied for synthesis of dinitrophenyl derivatives of nine amino alcohols and to work out a method of choice for sample preparation for direct enantioseparation. The enantiomeric dinitrophenyl derivatives so synthesized were separated on α 1-acid glycoprotein and β-cyclodextrin columns with detection at 230 nm using photodiode array detection system. Derivatization methods and chromatographic parameters were optimized. β-Cyclodextrin column was found better compared to AGP column for enantioseparation. Limit of detection, quantification, accuracy and precision were also determined. The developed method was successfully applied to determine enantiomeric purity of 1-amino-2-propanol obtained from vitamin B 12 hydrolysate.