Chemical Communications 2006-10-14

Dual colour, microarray-based, analysis of 10,000 protease substrates.

Juan J Díaz-Mochón, Laurent Bialy, Mark Bradley

Index: Chem. Commun. (Camb.) (38) , 3984-6, (2006)

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Abstract

A 10,000 member PNA-encoded library of FRET based peptides was synthesised for global analysis of protease cleavage specificity; analysis was achieved using a DNA microarray and consumed minimal quantities of enzyme (60 pmole) and library (3.5 nmole).