Biotechnology Letters 2012-01-01

Cholesterol assimilation and biotransformation by Lactobacillus helveticus.

Jayesh J Ahire, Anuja A Bhat, Jayashri M Thakare, Pallavi B Pawar, Dipali G Zope, Rakeshkumar M Jain, Bhushan L Chaudhari

Index: Biotechnol. Lett. 34(1) , 103-7, (2012)

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Abstract

Lactobacillus helveticus, grown at 37°C in MRS medium supplemented with 3 mM cholesterol, assimilated all the cholesterol in 42 h having 68 U mg(-1) of intracellular cholesterol oxidase activity. The strain transformed 1 g cholesterol to 0.05 g of androsta-1, 4-diene-3, 17-dione and 0.04 g of androst-4-ene-3, 17 dione within 48 h at 37°C with extracellular cholesterol oxidase activity at 12 U mg(-1) and intracellular oxidase at 0.5 U mg(-1).