Brain Research 1988-10-11

The effect of K+ and glutamate receptor agonists on the membrane potential of suspensions of primary cultures of rat astrocytes as measured with a cyanine dye, DiS-C2-(5).

M O Enkvist, I Holopainen, K E Akerman

Index: Brain Res. 462(1) , 67-75, (1988)

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Abstract

The cyanine dye DiS-C2-(5) was used to investigate the effect of K+ and glutamate receptor agonists on the membrane potential of whole populations of primary rat astrocytes in suspension. Increasing the external K+ concentration from 5 to 40 mM caused a depolarization of the cells. Ba2+ blocked the response to K+, whereas 4-aminopyridine had no effect on the depolarization. The effect of added external K+ was enhanced by the addition of the neutral K+ ionophore valinomycin. This supports the view that the membrane potential of primary astrocytes is dependent of the K+ gradient, and suggests that the membrane is not ideally permeable to K+ ions. Glutamate caused a depolarization of the cells which was not affected by Ba2+. In the presence of veratridine and ouabain no effect of glutamate was seen. The cells were also depolarized by the glutamate receptor agonists quisqualate, kainate and N-methyl-D-aspartate (NMDA). The response to kainate was blocked by kynurenate, which also diminished the depolarization caused by glutamate. NMDA was effective when added after kainate. The effect of the glutamate receptor agonists tested was generally smaller than that of glutamate itself, and a prior addition of one of the agonists diminished the response to glutamate. The results obtained suggest that cyanine dyes are well suited for investigating the behavior of whole populations of cultured primary astrocytes.