Biochimica et Biophysica Acta 1983-07-13

The application of a potential-sensitive cyanine dye to rat small intestinal brush border membrane vesicles.

B Stieger, G Burckhardt, H Murer

Index: Biochim. Biophys. Acta 732(1) , 324-6, (1983)

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Abstract

The sensitivity of the fluorescent dye, 3,3'-diethylthiadicarbocyanine (DiS-C2(5], was too low for the detection of membrane potential changes in rat small intestinal membrane vesicles. Only after adding LaCl3 or after fractionation of the intestinal membranes by free-flow electrophoresis could the dye be used to monitor electrogenic Na+-dependent transport systems. It is concluded that the response of this potential-sensitive dye is influenced by the negative surface charge density of the vesicles.