Phytochemical Analysis 2008-01-01

Validation of HPTLC method for the analysis of taraxerol in Clitoria ternatea.

Venkatesan Kumar, Kakali Mukherjee, Satheesh Kumar, Mainak Mal, Pulok K Mukherjee

Index: Phytochem. Anal. 19(3) , 244-50, (2008)

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Abstract

A new, simple, sensitive, selective and precise HPTLC method has been developed for the determination of taraxerol in Clitoria ternatea L. Determination of taraxerol was performed on TLC aluminium plates. Linear ascending development was carried out in twin trough glass chamber saturated with hexane and ethyl acetate (80:20 v/v). The plate was then dried and sprayed with anisaldehyde reagent. A Camag TLC scanner III was used for spectrodensitometric scanning and analysis at 420 nm. The system was found to give compact spots for taraxerol (R(f) 0.53). The calibration plot was linear in the range of 100-1200 ng of taraxerol. The correlation coefficient of 0.9961 was indicative of good linear dependence of peak area on concentration. The concentration of taraxerol was found to be 12.4 mg/g w/w in the hydroalcoholic extract of C. ternatea root. To study the accuracy and precision of the method, recovery studies were performed. Recovery values from 99.65 to 99.74% showed excellent reliability and reproducibility of the method. The limits of detection and quantification were determined to be 31 and 105 ng/spot, respectively. The proposed HPTLC method for quantitative monitoring of taraxerol in C. ternatea can be used for routine quality testing of C. ternatea extract used in Ayurvedic formulations.