Journal of AOAC International 2011-01-01

HPLC analysis of kaempherol and quercetin derivatives isolated by different extraction techniques from plant matrix.

Krystyna Skalicka-Woźniak, Janusz Szypowski, Kazimierz Głowniak

Index: J. AOAC Int. 94(1) , 17-21, (2011)

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Abstract

Soxhlet extraction, ultrasound extraction, and accelerated solvent extraction (ASE), followed by RP-HPLC with a photodiode array detector was used for the determination of flavonoids in fruits of Peucedanum alsaticum. Three compounds were identified: a kaempherol derivative (astragalin), and two quercetin derivatives (quercitrin and hiperoside). The highest extraction yields of the selected compounds were obtained by use of exhaustive ASE.