Analytical Biochemistry 2013-09-01

Fast mass spectrometry detection of tryptophan-containing peptides and proteins by reduction with pyridine-borane.

T Miron, M Wilchek

Index: Anal. Biochem. 440(1) , 12-4, (2013)

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Abstract

In this note, we describe a method devised to detect, by means of mass spectrometry (MS), tryptophan-containing peptides and proteins using pyridine-borane. This reagent selectively reduces tryptophan residues, converting them to 2,3-dihydro-tryptophan, thereby enabling quantitation of tryptophans. Copyright © 2013 Elsevier Inc. All rights reserved.