Molecular Pharmaceutics 2012-06-04

Lipase is essential for the study of in vitro release kinetics from organogels.

Marie-Hélène Dufresne, Elaheh Marouf, Yvonne Kränzlin, Marc A Gauthier, Jean-Christophe Leroux

Index: Mol. Pharm. 9(6) , 1803-11, (2012)

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Abstract

In vitro drug release studies remain indispensable in the development of drug delivery systems, even if correlations between in vitro and in vivo results are often imperfect. In this work, an improved in vitro analysis method for studying in situ-forming lipid-based implants was developed. More specifically, lipase was found to be an essential additive for evidencing differences in drug release kinetics from organogels of different amino acid-based organogelators, organogelator concentrations, drug loadings, and volumes. Lipases are thought to participate in the degradation of and release from amino acid-based organogel implants in vivo. Our experimental conditions allowed for the rapid and reliable screening of in vitro parameters that may be optimized to slow or accelerate drug release, once preliminary in vivo data are available.