Biosensors and Bioelectronics 2013-06-15

CdTe nanobioprobe based optoelectrochemical immunodetection of diabetic marker HbA1c.

Adity Chopra, Satish Tuteja, Naresh Sachdeva, K K Bhasin, Vijayender Bhalla, C Raman Suri

Index: Biosens. Bioelectron. 44 , 132-5, (2013)

Full Text: HTML

Abstract

Highly luminescent water soluble CdTe quantum dots (QDs) were synthesized and conjugated with anti-HbA1c antibody to generate specific nanobioprobe. A sandwich immunoassay model was employed using capture HbA1c antibody as a specific receptor molecule and the QD-labeled secondary antibody as a dual (fluorescence cum electrochemical) tracer to quantify the concentration of HbA1c. A linear increase in current was observed for HbA1c analytical standards with a R(2) value of 0.990 and coefficient of variance ~5%. A comparison between HPLC and dual immunoassay for clinical samples showed a correlation coefficient of 89% and 96% for fluorescence and electrochemical detection methods respectively. The QD-based immunoassay shows great promise for rapid reproducible and cost effective analysis of HbA1c in clinical samples.Copyright © 2013 Elsevier B.V. All rights reserved.