Analytica Chimica Acta 2007-03-14

Confirmation of four nitroimidazoles in porcine liver by liquid chromatography-tandem mass spectrometry.

X Xia, X Li, S Zhang, S Ding, H Jiang, J Shen

Index: Anal. Chim. Acta 586(1-2) , 394-8, (2007)

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Abstract

A sensitive and reliable multiresidue method is described for analysis of ronidazole, metronidazole, dimetridazole and the common metabolite of ronidazole and dimetridazole, 2-hydroxymethyl-1-methyl-5-nitroimidazole in swine liver. The sample preparation procedure was based on liquid-liquid extraction and mixed mode cation exchange/reverse phase solid-phase extraction. The compounds of interest were determined by reverse phase gradient liquid chromatography separation and tandem mass spectrometry (MS/MS) in the multiple reaction monitoring (MRM) mode. The limits of confirmation were 0.1-0.5 microg kg(-1) for the analytes.