Biomedical and Environmental Sciences 2011-08-01

The effect of 2,5-hexanedione on myelin protein zero expression, and its mitigation using Ginkgo biloba extract.

Lei Zhao, QingJun Liu, Hong Chen, HuaWei Duan, Ping Bin, Qing Liu, Yong Niu, YuFei Dai, YuXin Zheng, Lei ZHAO, QingJun LIU, Hong CHEN, HuaWei DUAN, Ping BIN, Qing LIU, Yong NIU, YuFei DAI, YuXin ZHENG

Index: Biomed. Environ. Sci. 24(4) , 374-82, (2011)

Full Text: HTML

Abstract

To investigate the role of myelin protein zero (P(0)) in 2,5-hexanedione (2,5-HD)-induced peripheral nerve injury, and the protective effect of Ginkgo biloba extract (Egb761) on 2,5-HD-induced toxic peripheral neuropathy.After 4 weeks of treatment with 2,5-HD at different doses (50, 100, 200, 400 mg/kg) in rats, changes in the levels of P(0) in rat sciatic nerves was investigated, and the effect of Egb761 on 2,5-HD-induced toxic peripheral neuropathy was studied.The blood-nerve barrier (BNB) permeability of the sciatic nerve increased, and the expression of P(0) mRNA and P(0) protein decreased in a dose-dependent manner after treatment with 2,5-HD for 4 weeks. Pretreatment with Egb761 protected against BNB interruption, and inhibited P(0) mRNA and protein reduction during 2,5-HD treatment. Pretreatment with Egb761 significantly reduced loss of body weight (P<0.01) and mitigated gait abnormalities (2.85±0.22) induced by 400 mg/kg 2,5-HD (P<0.01). It also reduced the signs of neurotoxicity induced by 2,5-HD.2,5-HD inhibited the expression of P(0) in a dose-dependent manner, and this may be an important mechanism by which toxic peripheral neuropathy is induced by 2,5-HD. Egb761 has a protective effect against 2,5-HD-induced peripheral neurotoxicity in rats.Copyright © 2011 The Editorial Board of Biomedical and Environmental Sciences. Published by Elsevier B.V. All rights reserved.