Lipids 1981-08-01

High performance reversed phase chromatography of cholesterol and cholesteryl esters of human plasma lipoproteins.

E G Perkins, D J Hendren, J E Bauer, A H El-Hamdy

Index: Lipids 16(8) , 609-13, (1981)

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Abstract

Cholesterol and cholesteryl esters were separated according to their carbon number and number of double bonds by high performance reversed-phase chromatography (HPRC) using acetonitrile/chloroform/methanol (1:1:1, v/v) as a mobile phase. It was found that within the same equivalent carbon number (ECN) category, cholesteryl esters with the highest number of double bonds eluted ahead of those with a lower number of double bonds, and with the cis isomers eluting ahead of their trans partners. Thus, cholesteryl oleate (C27-18:1c) elutes ahead of cholesteryl palmitate (C27-16:0) and ahead of cholesteryl elaidate (C27-18:1t). Human lipoprotein, as well as rat liver cholesteryl esters, were separated using this technique.