ChemBioChem 2011-12-16

Two-step, PCR-free telomerase detection by using exonuclease III-aided target recycling.

Xiaolei Zuo, Fan Xia, Adriana Patterson, H Tom Soh, Yi Xiao, Kevin W Plaxco

Index: ChemBioChem. 12(18) , 2745-7, (2011)

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Abstract

We report the sensitive detection of telomerase activity by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA-DNA molecular beacon. We demonstrate the specific detection of as few as 30 telomerase-positive breast cancer cells in a single-measurement fluorescence assay that avoids the problematic PCR and gel analysis of the current "gold-standard" assay.Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.