Chemico-Biological Interactions 1987-02-01

Allylic compounds bind directly to DNA: investigation of the binding mechanisms in vitro.

E Eder, D Lutz, M Jörns

Index: Chem. Biol. Interact. 61(2) , 97-108, (1987)

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Abstract

The in vitro binding of three allyl compounds, allyl methanesulphonate, allyl bromide and allyl chloride to DNA from salmon sperm was investigated. Kinetic DNA-alkylation measurements revealed significant differences between the strongly alkylating allyl methanesulphonate and allyl bromide with half-life values of 1.5 h and 8.1 h, respectively and the weakly alkylating allyl chloride with a half-life value of 360 h. Five alkylated nucleic bases, 3-allyladenine, N6-allyladenine, N2-allylguanine, 7-allylguanine and O6-allylguanine, could be identified after DNA-alkylation with all three allyl compounds.