Bioanalysis 2016-04-01

Polar stir bars for isolation and preconcentration of perfluoroalkyl substances from human milk samples prior to UHPLC-MS/MS analysis.

Julia Martín, Rocío Rodríguez-Gómez, Alberto Zafra-Gómez, Esteban Alonso, José L Vílchez, Alberto Navalón

Index: Bioanalysis 8 , 633-47, (2016)

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Abstract

A new method for the determination of four perfluoroalkyl carboxylic acids (from C5 to C8) and perfluorooctane sulfonate in human milk samples using stir-bar sorptive extraction-ultra-HPLC-MS/MS has been accurately optimized and validated.Polydimethylsiloxane and polyethyleneglycol modified silicone materials were evaluated.Overall, polyethyleneglycol led to a better sensitivity. After optimizing experimental variables, the method was validated reaching detection limits in the range of 0.05-0.20 ng ml(-1); recovery rates from 81 to 105% and relative standard deviations fewer than 13% in all cases. The method was applied to milk samples from five randomly selected women. All samples were positive for at least one of the target compounds with concentrations ranging between 0.8 and 6.6 ng ml(-1), being the most abundant perfluorooctane sulfonate.