Analytical Biochemistry 1993-11-01

One-step conversion of amino acids into N-menthyloxycarbonyl alkyl ester derivatives for chiral gas chromatography.

N Domergue, M Pugniere, A Previero

Index: Anal. Biochem. 214(2) , 420-5, (1993)

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Abstract

Amino acids dissolved in aqueous methanol (or ethanol) and treated with optically active menthyl chloroformate were converted into N-menthyloxycarbonyl methyl (or ethyl) ester derivatives within a few minutes at room temperature. The obtained diastereomeric derivatives, with the exception of arginine and histidine, had suitable gas chromatographic properties allowing enantiomeric analysis of a large number of proteinogenic and synthetic amino acids.