Biomaterials 2013-06-01

An injectable cell penetrable nano-polyplex hydrogel for localized siRNA delivery.

Young-Min Kim, Mi-Ran Park, Soo-Chang Song

Index: Biomaterials 34(18) , 4493-500, (2013)

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Abstract

An approach for application of cell penetration to selective small interference RNA (siRNA) localized delivery system, cell penetrable nano-polyplex assembled hydrogel system, is presented. The cell penetrable nano-polyplex assembled hydrogelisprepared by protamine conjugation to poly(organophos-phazene) and inducement of nano-polyplexes with siRNAs. After an injection of cell penetrable nano-polyplex solution into the body, it turns into a gel due to thermosensitivity of poly(organo- phosphazene). The gel maintains up to 4 weeks and the released 30 nm-sized nano-polyplexes from the gel induces highly effective siRNA delivery due to cell penetration. Accordingly, the new system shows a high gene silencing efficiency on only the target site in long-term with a single injection.Copyright © 2013 Elsevier Ltd. All rights reserved.