Journal of Chromatography B 2014-08-15

Quantification of five plasticizers used in PVC tubing through high performance liquid chromatographic-UV detection.

Tsanta Radaniel, Stéphanie Genay, Nicolas Simon, Frédéric Feutry, Francesca Quagliozzi, Christine Barthélémy, Marie Lecoeur, Valérie Sautou, Bertrand Décaudin, Pascal Odou

Index: J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 965 , 158-63, (2014)

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Abstract

Searching for alternatives to di-(2-ethylhexyl)-phthalate, a plasticizer that has been widely used in the manufacturing of PVC medical devices, has become a major challenge since a European regulation underlined some clinical risks. The aim of this study is to develop an HPLC-UV method to quantify the currently used alternative plasticizers to DEHP. Five plasticizers, acetyl tributyl citrate, di-(2-ethylhexyl)-phthalate, di-(ethylhexyl)-terephthalate, di-isononyl-1,2-cyclohexane-dicarboxylate, and trioctyl trimellitate, were separated on a C8 stationary phase (2.6 μm, 100 mm × 4.6mm) under gradient elution in 13 min. They were detected at 221 nm leading to a quantification threshold from 0.3 to 750 μg/mL as a function of the plasticizer. Within-day and between-day precisions were inferior to 0.9% and 18%, respectively. The assays were validated according to the accuracy profile method. Plasticizers were extracted from PVC-tubing by dissolving PVC in THF then precipitating it in methanol with a yield of over 90% for each plasticizer. This assay could feasibly be used to quantify plasticizers in PVC medical devices.Copyright © 2014 Elsevier B.V. All rights reserved.