Chemical Communications 2010-03-28

5-Carboxyfluorescein tagged N-phenylanthranilamide as a tracer reagent for fluorescence polarization: a robust method to screen MAPK pathway allosteric inhibitors.

Z Nooshin Rezvani, R John Mayer, Weng C Chan

Index: Chem. Commun. (Camb.) 46(12) , 2043-5, (2010)

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Abstract

Fluorescence polarization using N(alpha)-(fluorescein-5-carbonyl)-N(epsilon)-(N-[2-fluoro-4-iodophenyl]-3,4-difluoroanthraniloyl)lysyl amide is capable of rapidly identifying inhibitor ligands that bind to an allosteric site in MEK1.