Journal of Pharmaceutical and Biomedical Analysis 2015-12-10

Enantioselective analysis of D- and L-amino acids from mouse macrophages using high performance liquid chromatography.

Shiro Kato, Yuki Masuda, Morichika Konishi, Tadao Oikawa

Index: J. Pharm. Biomed. Anal. 116 , 101-4, (2015)

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Abstract

The intrinsic D-amino acid profile of mouse macrophages extracted from the peritoneal cavity was analyzed using high performance liquid chromatography. Six D-amino acids (D-Asp, D-Ser, D-Ala, D-Leu, D-Gln and D-Lys) were detected in cell lysates of mouse macrophages. The content and the D/D+L ratio differed depending on the type of D-amino acid and were approximately 3.5-22 nmol/g cells, and approximately 1-20%, respectively. The D-amino acid composition of RAW 264.7 cells, which is a model macrophage cell line, was similar to that of the mouse macrophage. These results suggest that macrophages and RAW 264.7 cells with macrophage-like functions have a similar D-amino acid profile.Copyright © 2015 Elsevier B.V. All rights reserved.