Journal of Immunological Methods 2015-01-01

High affinity anti-Internalin B VHH antibody fragments isolated from naturally and artificially immunized repertoires.

Robert W Gene, Jyothi Kumaran, Cristina Aroche, Henk van Faassen, J Christopher Hall, C Roger MacKenzie, Mehdi Arbabi-Ghahroudi

Index: J. Immunol. Methods 416 , 29-39, (2015)

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Abstract

The need for rapid and easy technologies for the detection of food-borne and environmental pathogens is essential for safeguarding the health of populations. Furthermore, distribution of tainted food and water can have consequences which can affect whole economies. Antibodies and antibody fragments have been historically used in detection platforms due to their antigen specificity and robust physicochemical properties. In this study, we report the isolation and characterization of antibody fragments from the heavy chain antibody repertoire (VHH) of Camelidae which bind with specificity and high affinity to the Listeria monocytogenes invasin, Internalin B (InlB). To the best of our knowledge, this is the first report of anti-InlB VHHs from camelids. These anti-InlB VHHs were not cross-reactive to the structurally related Listeria invasin Internalin A (InlA) and are potential reagents to be used in the development of detection and medical technologies. Copyright © 2014. Published by Elsevier B.V.