FEBS Letters 2014-12-20

Kinetics of the early events of GPCR signalling.

Roslin J Adamson, Anthony Watts

Index: FEBS Lett. 588(24) , 4701-7, (2014)

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Abstract

Neurotensin receptor type 1 (NTS1) is a G protein-coupled receptor (GPCR) that affects cellular responses by initiating a cascade of interactions through G proteins. The kinetic details for these interactions are not well-known. Here, NTS1-nanodisc-Gαs and Gαi1 interactions were studied. The binding affinities of Gαi1 and Gαs to NTS1 were directly measured by surface plasmon resonance (SPR) and determined to be 15±6 nM and 31±18 nM, respectively. This SPR configuration permits the kinetics of early events in signalling pathways to be explored and can be used to initiate descriptions of the GPCR interactome.Copyright © 2014 The Authors. Published by Elsevier B.V. All rights reserved.