Analytical and Bioanalytical Chemistry 2015-09-01

Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach.

Rita C Alves, Filipa B Pimentel, Henri P A Nouws, Wagner Correr, María Begoña González-García, M Beatriz P P Oliveira, Cristina Delerue-Matos

Index: Anal. Bioanal. Chem 407 , 7157-63, (2015)

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Abstract

A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8%) and accuracy (recoveries ≥96.7%). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.