Journal of Pharmaceutical and Biomedical Analysis 2014-11-01

Liquid chromatography-mass spectrometry based serum peptidomic approach for renal clear cell carcinoma diagnosis.

Zhenzhen Huang, Shudi Zhang, Wei Hang, Yuedong Chen, Jiaxin Zheng, Wei Li, Jinchun Xing, Jie Zhang, Eryi Zhu, Xiaomei Yan

Index: J. Pharm. Biomed. Anal. 100 , 175-83, (2014)

Full Text: HTML

Abstract

Serum peptidomic approach was applied to investigate the peptidomic signature and discover the clinical biomarkers and biomarker patterns for RCC patients. The holistic orthogonal partial least-squares-discriminant analysis (OPLS-DA) based on qualified profile data successfully classified RCC patients from healthy controls, showing 100% sensitivity and specificity. Following critical criteria, several peptides presenting significant differences in serum level were picked out. The unsupervised hierarchical cluster analysis on those peptides was performed, showing 100% sensitivity and 93.3% specificity for RCC diagnosis regarding the present samples. Besides, receiver-operating characteristic (ROC) analysis was applied on single peptide biomarkers, with four peptides showing excellent predictive power. Among them, IYQLNSKLV and AGISMRSGDSPQD are reported for the first time for cancer detection. Copyright © 2014 Elsevier B.V. All rights reserved.