Applied and Environmental Microbiology 2013-10-01

Clostridium acidurici electron-bifurcating formate dehydrogenase.

Shuning Wang, Haiyan Huang, Jörg Kahnt, Rudolf K Thauer

Index: Appl. Environ. Microbiol. 79(19) , 6176-9, (2013)

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Abstract

Cell extracts of uric acid-grown Clostridium acidurici catalyzed the coupled reduction of NAD(+) and ferredoxin with formate at a specific activity of 1.3 U/mg. The enzyme complex catalyzing the electron-bifurcating reaction was purified 130-fold and found to be composed of four subunits encoded by the gene cluster hylCBA-fdhF2.