Neurochemical Research 2015-08-01

Monitoring newly synthesized proteins over the adult life span of Caenorhabditis elegans.

Peiling Ben, Zhengping Zhang, Chunxia Xuan, Shasha Sun, Lei Shen, Yanhong Gao, Xiang Cao, Yi Zhou, Lei Lan, Zhimin Yin, Lan Luo

Index: J. Proteome Res. 14(3) , 1483-94, (2015)

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Abstract

L-Theanine is an amino acid derivative from green tea. The present work was aimed at the effect of L-theanine on neuron-like rat pheochromocytoma (PC12) cells stimulated with cadmium chloride. Treatment with L-theanine before cadmium exposure increased cell viability; the experiments of Annexin V/PI staining indicated that L-theanine inhibited cadmium-induced cell apoptosis. Meanwhile, L-theanine decreased ROS production and protected from cadmium-induced disruption of mitochondrial transmembrane potential. Compared with cadmium-treated cells, L-theanine could also decrease the ratio of Bax/Bcl-2, as well as the level of cleaved caspase-9, caspase-3 and poly(ADP-ribose) polymerase. Furthermore, L-theanine depresses cadmium-induced up regulation of phosphorylations of PI3K/Akt, MAPK ERK1/2, and JNK signaling. These data suggest that L-theanine pretreatment reduces severity of cadmium toxicity probably via antioxidant action. Therefore, it may be concluded that L-theanine could be exploited for prevention of cadmium-induced diseases.