Bioscience, Biotechnology, and Biochemistry 2013-01-01

Characterization of an α-L-Rhamnosidase from Streptomyces avermitilis.

Hitomi Ichinose, Zui Fujimoto, Satoshi Kaneko

Index: Biosci. Biotechnol. Biochem. 77(1) , 213-6, (2013)

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Abstract

The putative α-L-rhamnosidase gene from Streptomyces avermitilis was cloned and expressed. The recombinant enzyme released L-rhamnose from p-nitrophenyl α-L-rhamnoside, Citrus flavonoids such as naringin, rutin, and hesperidin, and gum arabic which is an arabinogalactan-protein. Calcium ions increased L-rhamnose production by the enzyme from gum arabic, whereas enzyme activity was not affected by any metal ions.