Carbohydrate Research

Determination of the transgalactosylation activity of Aspergillus oryzae β-galactosidase: effect of pH, temperature, and galactose and glucose concentrations

C Vera, C Guerrero, A Illanes

Index: Vera, Carlos; Guerrero, Cecilia; Illanes, Andres Carbohydrate Research, 2011 , vol. 346, # 6 p. 745 - 752

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Citation Number: 43

Abstract

The catalytic potential of β-galactosidase is usually determined by its hydrolytic activity over natural or synthetic substrates. However, this method poorly predicts enzyme behavior when transglycosylation instead of hydrolysis is being performed. A system for determining the transgalactosylation activity of β-galactosidase from Aspergillus oryzae was developed, and its activity was determined under conditions for the synthesis of galacto-oligosaccharides ...