e.g. Filippa Pettersson or Cancer Res. 75(6) , 1102-12, (2015) or 10.1002/anie.201600521
Tetrahedron letters
Chemoenzymatic one-pot synthesis of cefazolin from cephalosporin C in fully aqueous medium, involving three consecutive biotransformations catalyzed by D- …
R Fernández-Lafuente, JM Guisán, M Pregnolato…
Index: Fernandez-Lafuente, Roberto; Guisan, Jose M.; Pregnolato, Massimo; Terreni, Marco Tetrahedron Letters, 1997 , vol. 38, # 26 p. 4693 - 4696
A new chemoenzymatic synthesis of Cefazolin through the correct assembly of three biotransformations catalysed by D-aminoacid oxidase, glutaryl acylase and penicillin G acylase is described. This multienzymatic synthesis has been performed from the natural Cephalosporin C in fully aqueous medium without intermediate purification stages. Almost quantitative yields have been achieved in all the enzymatic reactions.
