Tetrahedron Letters

Enzymatic formation of unnatural novel polyketide scaffolds by plant-specific type III polyketide synthase

SP Shi, H Morita, K Wanibuchi, H Noguchi, I Abe

Index: Shi, She-Po; Morita, Hiroyuki; Wanibuchi, Kiyofumi; Noguchi, Hiroshi; Abe, Ikuro Tetrahedron Letters, 2009 , vol. 50, # 18 p. 2150 - 2153

Full Text: HTML

Citation Number: 4

Abstract

The catalytic potential of octaketide synthase (OKS), a plant-specific type III polyketide synthase (PKS) from Aloe arborescens, was investigated by phenylacetyl-CoA and benzoyl- CoA as starter substrates. As a result, a novel C16 pentaketide coumarin was produced from phenylacetyl-CoA, whereas benzoyl-CoA was not a good substrate of OKS. Remarkably, a structure-guided OKS N222G mutant dramatically extended the product chain length to ...