We report the synthesis and guest affinities of polymers featuring a single recognition site in the middle of the polymer chain. These polymers were synthesized from difunctional initiators based on the 2, 6-diacyldiaminopyridine moiety. Binding efficiencies were experimentally determined using a complementary fluorescent guest, N (10)-isopropyl flavin. The effect of polymer length on recognition was explored by the synthesis of 5 PMMA ...