Tetrahedron

The action of adenosine deaminase (EC 3.5. 4.4.) on adenosine and deoxyadenosine acetates: the crucial role of the 5′-hydroxy group for the enzyme activity

P Ciuffreda, S Casati, E Santaniello

Index: Ciuffreda, Pierangela; Casati, Silvana; Santaniello, Enzo Tetrahedron, 2000 , vol. 56, # 20 p. 3239 - 3243

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Citation Number: 26

Abstract

From adenosine 1, 2′-deoxyadenosine 3 and 3′-deoxyadenosine 5 all the acetates were prepared by lipase-catalyzed reactions. Only the acetates with free 5′-hydroxy group were deaminated by adenosine deaminase (ADA), confirming the crucial role of 5′-OH for the enzyme activity.