... Tetrahedron: Asymmetry. Volume 15, Issue 10, 24 May 2004, Pages 1551–1554. Cover image Cover image. ... To diethyl 3-hydroxyglutarate 3 (3.0 g, 14.7 mmol) was added buffer (15 mL) and CALB (0.5 and 0.05 g) and the reaction monitored using a pH-stat. ...