Phytochemistry

Molecular cloning, expression, and characterization of adenylate isopentenyltransferase from hop (Humulus lupulus L.)

…, A Matsunaga, T Suwama, T Kaneko, K Ito, H Noguchi…

Index: Sakano, Yuichi; Okada, Yukio; Matsunaga, Akiko; Suwama, Takaharu; Kaneko, Takafumi; Ito, Kazutoshi; Noguchi, Hiroshi; Abe, Ikuro Phytochemistry, 2004 , vol. 65, # 17 p. 2439 - 2446

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Citation Number: 36

Abstract

A cDNA encoding adenylate isopentenyltransferase (AIPT) was cloned and sequenced from cones of hop (Humulus lupulus L.) by RT-PCR using oligonucleotide primers based on the conserved sequences of Arabidopsis thaliana AIPT isozymes (AtIPT1, AtIPT3, AtIPT4, AtIPT5, AtIPT6, AtIPT7 and AtIPT8). A full-length cDNA contained a 990-bp open reading frame encoding a molecular mass of 36,603 Da protein with 329 amino acids. Further, ...