Bioorganometallic mechanism of action, and inhibition, of IspH

W Wang, K Wang, YL Liu, JH No, J Li…

Index: Wang, Weixue; Wang, Ke; Liu, Yi-Liang; No, Joo-Hwan; Li, Jikun; Nilges, Mark J.; Oldfield, Eric Proceedings of the National Academy of Sciences of the United States of America, 2010 , vol. 107, # 10 p. 4522 - 4527

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Citation Number: 75

Abstract

Abstract We have investigated the mechanism of action of Aquifex aeolicus IspH [E-4- hydroxy-3-methyl-but-2-enyl diphosphate (HMBPP) reductase], together with its inhibition, using a combination of site-directed mutagenesis (KM, V max), EPR and 1 H, 2 H, 13 C, 31 P, and 57 Fe-electron-nuclear double resonance (ENDOR) spectroscopy. On addition of HMBPP to an (unreactive) E126A IspH mutant, a reaction intermediate forms that has a ...

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