The novel antifungal karnamicin B1 (1), 5-hydroxy-3, 4-dimethoxy-2-{2-(4-oxopentyl)-4- thiazolyl} pyridine-6-carboxamide was first synthesized via the formation of partially methylated trihydroxy pyridine by ring-transformation from the corresponding furan derivative and subsequent regioselective introduction of 2, 6-substituents using Meisenheimer-type reaction.