2-(Benzothiazol-2-yl)-phenyl-β-D-galactopyranoside derivatives as fluorescent pigment dyeing substrates and their application for the assay of β-D-galactosidase …

…, R Taguchi, T Takahashi, T Suzuki, F Teraoka…

Index: Otsubo, Tadamune; Minami, Akira; Fujii, Haruna; Taguchi, Risa; Takahashi, Tadanobu; Suzuki, Takashi; Teraoka, Fumiteru; Ikeda, Kiyoshi Bioorganic and Medicinal Chemistry Letters, 2013 , vol. 23, # 7 p. 2245 - 2249

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Citation Number: 15

Abstract

2-(Benzothiazol-2-yl)-phenyl-β-d-galactopyranoside derivatives were synthesized as novel artificial fluorescent pigment dyeing substrates for β-d-galactosidase. The substrates, which exhibited non-fluorescence or weak fluorescence in solution phase, were smoothly hydrolyzed by β-d-galactosidase from Aspergillus oryzae and yielded a water-insoluble strong fluorescent pigment. The difference of fluorescent intensity exhibited a linear ...