Imaging of conformational changes of proteins with a new environment-sensitive fluorescent probe designed for site-specific labeling of recombinant proteins in live …

J Nakanishi, T Nakajima, M Sato, T Ozawa…

Index: Nakanishi; Nakajima; Sato; Ozawa; Tohda; Umezawa Analytical Chemistry, 2001 , vol. 73, # 13 p. 2920 - 2928

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Citation Number: 84

Abstract

We demonstrate herein a new method for imaging conformational changes of proteins in live cells using a new synthetic environment-sensitive fluorescent probe, 9-amino-6, 8-bis (1, 3, 2-dithioarsolan-2-yl)-5H-benzo [a] phenoxazin-5-one. This fluorescent probe can be attached to recombinant proteins containing four cysteine residues at the i, i+ 1, i+ 4, and i+ 5 positions of an α-helix. The specific binding of the fluorescent probe to this 4Cys motif ...