Chemistry & biology

Deciphering the late biosynthetic steps of antimalarial compound FR-900098

TW Johannes, MA DeSieno, BM Griffin, PM Thomas…

Index: Johannes, Tyler W.; DeSieno, Matthew A.; Griffin, Benjamin M.; Thomas, Paul M.; Kelleher, Neil L.; Metcalf, William W.; Zhao, Huimin Chemistry and Biology, 2010 , vol. 17, # 1 p. 57 - 64

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Citation Number: 20

Abstract

FR-900098 is a potent chemotherapeutic agent for the treatment of malaria. Here we report the heterologous production of this compound in Escherichia coli by reconstructing the entire biosynthetic pathway using a three-plasmid system. Based on this system, whole-cell feeding assays in combination with in vitro enzymatic activity assays reveal an unusual functional role of nucleotide conjugation and lead to the complete elucidation of the ...