Detection of in vivo matrix metalloproteinase activity using microdialysis sampling and liquid chromatography/mass spectrometry

…, DV Zagorevski, MR Lennartz, DJ Loegering…

Index: Wang, Ying; Zagorevski, Dmitri V.; Lennartz, Michelle R.; Loegering, Daniel J.; Stenken, Julie A. Analytical Chemistry, 2009 , vol. 81, # 24 p. 9961 - 9971

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Citation Number: 21

Abstract

Matrix metalloproteinases (MMPs) are a family of endoproteases that break down extracellular matrix and whose upregulation contributes to several diseases. A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed to quantify MMP-1 and MMP-9 substrates and their N-terminal peptide products in samples obtained from implanted microdialysis sampling probes. In vitro studies with purified human MMP-1 ...