Enantioselectivity of haloalkane dehalogenases and its modulation by surface loop engineering

…, R Chaloupkova, T Koudelakova, P Jerabek…

Index: Prokop, Zbynek; Sato, Yukari; Brezovsky, Jan; Mozga, Tomas; Chaloupkova, Radka; Koudelakova, Tana; Jerabek, Petr; Stepankova, Veronika; Natsume, Ryo; Van Leeuwen, Jan G. E.; Janssen, Dick B.; Florian, Jan; Nagata, Yuji; Senda, Toshiya; Damborsky, Jiri Angewandte Chemie - International Edition, 2010 , vol. 49, # 35 p. 6111 - 6115

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Citation Number: 74

Abstract

Enzymes are widely used for the synthesis of pharmaceuticals, agrochemicals, and food additives because they can catalyze enantioselective transformations.[1] Understanding the molecular basis of enzyme–substrate interactions that contribute to enantioselectivity is important for constructing selective enzymes by protein engineering.[2] Up to now, emphasis has been on reactions such as lipase-or esterase-based kinetic resolutions,[2d, 3] as well ...