Purification, characterization, and gene identification of an α-glucosyl transfer enzyme, a novel type α-glucosidase from Xanthomonas campestris WU-9701

…, N Hasegawa, J Saito, S Umezawa, Y Honda…

Index: Sato, Toshiyuki; Hasegawa, Nobukazu; Saito, Jun; Umezawa, Satoru; Honda, Yuki; Kino, Kuniki; Kirimura, Kohtaro Journal of Molecular Catalysis B: Enzymatic, 2012 , vol. 80, p. 20 - 27

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Citation Number: 8

Abstract

The α-glucosyl transfer enzyme (XgtA), a novel type α-glucosidase produced by Xanthomonas campestris WU-9701, was purified from the cell-free extract and characterized. The molecular weight of XgtA is estimated to be 57kDa by SDS-PAGE and 60kDa by gel filtration, indicating that XgtA is a monomeric enzyme. Kinetic properties of XgtA were determined for α-glucosyl transfer and maltose-hydrolyzing activities using ...