The application of Lipase enzymes to effect regioselective C-3-O-acylation of 4, 6-O- benzylidene-β-d-gluco-and-galactopyranosides displaying a range of anomeric substituents, and C-2-O-acylation of phenyl 4, 6-O-benzylidene-α-d-glucopyranoside and ethyl 4, 6-O-benzylidene-1-thio-α-d-glucopyranoside is reported. In particular this method has allowed introduction of a variety of acyl protecting groups at the C-3 hydroxyl group of ...