Overexpression of a plant cysteine synthase gene and biosynthesis of a plant specific metabolite, β-(pyrazol-1-yl)-L-alanine, in Escherichia coli

K Saito, R Kanda, M Kurosawa…

Index: Saito; Kanda; Kurosawa; Murakoshi Canadian Journal of Chemistry, 1994 , vol. 72, # 1 p. 188 - 192

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Citation Number: 4

Abstract

Cysteine synthase (EC 4.2. 99.8) in higher plants is responsible for biosynthesis of not only cysteine but also some nonprotein amino acids such as β-(pyrazol-1-yl)-l-alanine. The cDNA of a cysteine synthase from spinach (Spinacia oleracea) was inserted into pET8c (= pET3d) under the transcriptional control of strong T7 promoter to yield an overexpression vector pCEK1. The amount of the exogenous cysteine synthase was increased up to 40% of the ...