A fluorescence sensor for detection of geranyl pyrophosphate by the chemo-ensemble method

KH Chen, JH Liao, HY Chan…

Index: Chen, Kuan-Hung; Liao, Jen-Hai; Chan, Hsin-Yu; Fang, Jim-Min Journal of Organic Chemistry, 2009 , vol. 74, # 2 p. 895 - 898

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Citation Number: 30

Abstract

Hexaamide receptor 4 and coumarin phosphate 5 form an ensemble for effective sensing of geranyl pyrophosphate (GPP). Fluorescence resonance energy transfer in the 4+ 5 ensemble diminishes when 5 is replaced by GPP. Receptor 4 binds selectively with GPP over other anions, including fatty acids. The 4− GPP complex in 1: 1 stoichiometry is inferred by NMR and fluorescence titrations. Receptor 4 contains a pseudotetrahedral cleft to ...